Mouse Genetics Laboratory

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Mouse Genetics Laboratory (MGL), acting as a Research Resource for the Academic Health Center, provides a comprehensive range of mouse embryo manipulation services.

Housed in the Cancer Cardiovascular Research Building (CCRB), MGL offers microinjection, mouse embryo manipulation, tissue culture, and administration capabilities in conjunction with dedicated animal housing space. 

Service Overview

ServiceDescriptionCost Estimate
DNA or CRISPR transgenicsDirect zygote microinjection$4545 (FVB) or $5050 (non-FVB) + per diem
ES cell injection to make chimera miceES cell injection into blastocyst $2360/mutation + per diem
Sperm cryoCryopreservation of sperm$505/1-3 lines
In Vitro Fertilization (IVF)Fertilization of eggs and embryo transfer$1655/line + per diem
Recover mice from frozen embryoEmbryo transfer$505 + mouse cost + per diem
Special serviceProject specific, procedure varies.$505/day + mouse cost + per diem

Down load the Request for Services form

Down Load the Request for Service form

Service and cost details

Overview video

Mouse Genetics - Masonic Cancer Center

Chimera mouse with an albino mouse

Creating mutant mice by CRISPR/Cas9 injection

Working with Genome Editing Shared Resources (GESR) MGL can provide a full service for your CRISPR/Cas9 project to produce mutant mice.  

To start, please contact either MGL coordinator Yun You (yyou@umn.edu or GESR coordinator Brian Ruis (ruisx001@umn.edu.

Note: CRISPR/Cas9 approach is the method of choice to make simple gene knock-out.  It is also very useful to generate point mutations. However, it is still a work in progress to efficiently insert two LoxP elements to flank a genomic region (conditional allele) or insert large DNA fragment (GFP, etc.). Please contact MGL to discuss your project.

 Contact us

Timothy Hallstrom, Ph.D., Director
612-626-2905
halls026@umn.edu

Yun You, Ph.D., Coordinator
612-626-5825
yyou@umn.edu

Location:

Mouse Genetics Laboratory (MGL)
Cancer and Cardiovascular Research Building
2231 6th St. SE
Minneapolis, MN, 55455