Mouse Genetics Laboratory
Mouse Genetics Laboratory (MGL), acting as a Research Resource for the Academic Health Center, provides a comprehensive range of mouse embryo manipulation services.
Housed in the Cancer Cardiovascular Research Building (CCRB), MGL offers microinjection, mouse embryo manipulation, tissue culture, and administration capabilities in conjunction with dedicated animal housing space.
Service & Cost Overview
|Service||Description||Non-Cancer Center Member Cost Estimate||Cancer Center Member Cost Estimate|
|CRISPR or DNA transgenics||Direct zygote microinjection||$4545 (FVB) or $5050 (non-FVB) + per diem||$2272.50 (FVB) or $2525 (non-FVB) + per diem|
|ES cell injection to make chimera mice||ES cell injection into blastocyst||$2360/mutation + per diem||$1180/mutation + per diem|
|Sperm cryo||Cryopreservation of sperm||$505/1-3 lines||$252.50/1-3 lines|
|In Vitro Fertilization (IVF)||Fertilization of eggs and embryo transfer||$1655/line + per diem||$827.50/line + per diem|
|Recover mice from frozen embryo||Embryo transfer||$505 + mouse cost + per diem||$252.50 + mouse cost + per diem|
|Special service||Project specific, procedure varies.||$505/day + mouse cost + per diem||$252.50/day + mouse cost + per diem|
Creating mutant mice by CRISPR/Cas9 injection
Note: CRISPR/Cas9 approach is the method of choice to make simple gene knock-out and precision point mutations. More recently, substancial strides were made to enhance the rate of success in making conditional alleles (inserting LoxP, Frt, etc). Please contact MGL to discuss your project.