Mouse Genetics Laboratory

Chimera mouse with 2 different eye color

Mouse Genetics Laboratory (MGL), acting as a Research Resource for the Academic Health Center, provides a comprehensive range of mouse embryo manipulation services.

Housed in the Cancer Cardiovascular Research Building (CCRB), MGL offers microinjection, mouse embryo manipulation, tissue culture, and administration capabilities in conjunction with dedicated animal housing space. 

Download the Request for Services form

Download the Request for Service form

Service & Cost Overview

ServiceDescriptionNon-Cancer Center Member Cost EstimateCancer Center Member Cost Estimate
CRISPR or DNA transgenicsDirect zygote microinjection$4545 (FVB) or $5050 (non-FVB) + per diem$2272.50 (FVB) or $2525 (non-FVB) + per diem
ES cell injection to make chimera miceES cell injection into blastocyst $2360/mutation + per diem$1180/mutation + per diem
Sperm cryoCryopreservation of sperm$505/1-3 lines$252.50/1-3 lines
In Vitro Fertilization (IVF)Fertilization of eggs and embryo transfer$1655/line + per diem$827.50/line + per diem
Recover mice from frozen embryoEmbryo transfer$505 + mouse cost + per diem$252.50 + mouse cost + per diem
Special serviceProject specific, procedure varies.$505/day + mouse cost + per diem$252.50/day + mouse cost + per diem

Overview video

Mouse Genetics - Masonic Cancer Center

Creating mutant mice by CRISPR/Cas9 injection

Working with Genome Engineering Shared Resource (GESR) MGL can provide a full service for your CRISPR/Cas9 project to produce mutant mice.  

Work flow and cost

To start, please contact either MGL coordinator Yun You (yyou@umn.edu or GESR coordinator Brian Ruis (ruisx001@umn.edu.

 

Note: CRISPR/Cas9 approach is the method of choice to make simple gene knock-out and precision point mutations. More recently, substancial strides were made to enhance the rate of success in making conditional alleles (inserting LoxP, Frt, etc). Please contact MGL to discuss your project.

Contact us

Timothy Hallstrom, Ph.D., Director
612-626-2905
halls026@umn.edu

Yun You, Ph.D., Coordinator
612-626-5825
yyou@umn.edu

Location:

Mouse Genetics Laboratory (MGL)
Cancer and Cardiovascular Research Building
2231 6th St. SE
Minneapolis, MN, 55455