Mouse Genetics Laboratory
Mouse Genetics Laboratory (MGL), acting as a Research Resource for the Academic Health Center, provides a comprehensive range of mouse embryo manipulation services.
Housed in the Cancer Cardiovascular Research Building (CCRB), MGL offers microinjection, mouse embryo manipulation, tissue culture, and administration capabilities in conjunction with dedicated animal housing space.
Service & Cost Overview
|Service||Description||Non-Cancer Center Member Cost Estimate||Cancer Center Member Cost Estimate|
|CRISPR or DNA transgenics||Direct zygote injection or electroporation||$4545 (FVB) or $5050 (non-FVB) + per diem||$2272.50 (FVB) or $2525 (non-FVB) + per diem|
|ES cell injection to make chimera mice||ESC or iPSC injection into blastocyst||$2360/mutation + per diem||$1180/mutation + per diem|
|Sperm cryo||Cryopreservation of sperm||$505/1-3 lines/PI (Lab)||$252.50/1-3 lines/PI (Lab)|
|In Vitro Fertilization (IVF)||Fertilization of eggs and embryo transfer||$1655/line + per diem||$827.50/line + per diem|
|Recover mice from frozen embryo||Embryo transfer||$505 + mouse cost + per diem||$252.50 + mouse cost + per diem|
|Special service||Project specific, procedure varies.||$505/day + mouse cost + per diem||$252.50/day + mouse cost + per diem|
Creating mutant mice by CRISPR/Cas9 methods
Working with Genome Engineering Shared Resource (GESR) MGL can provide full service for your CRISPR/Cas9 project to produce mutant mice. We have successfully created mutant mice with simple gene KO (indels), large deletions (more than 1 Kb), point mutations, conditional KO alleles (inserting 2 LoxP elements), and knock-in DNA fragments (> 4 Kb).
Note: CRISPR/Cas9 approach is the method of choice to make simple gene knock-out and precision point mutations. More recently, substancial strides were made to enhance the rate of success in making conditional alleles (inserting LoxP, Frt, etc). Please contact MGL to discuss your project.