Mouse Genetics Laboratory

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The University of Minnesota's Mouse Genetics Laboratory (MGL), acting as a Research Resource for the Academic Health Center provides a comprehensive range of mouse embryo manipulation services.

Housed in the Cancer Cardiovascular Research Building (CCRB), the Mouse Genetics Laboratory offers a micro-injection laboratory, tissue culture, and administration capabilities in conjunction with dedicated animal housing space.


Services

Transgenic mouse production by pronuclear (DNA) or cytoplasm (CRISPR/Cas9) injection

Knockout mouse production by blastocyst injection

Sperm cryopreservatiosn

In vitro fertilization (IVF)

Special services


Request for Services form

Submit your Request for Service form along with the appropriate data sheet (listed below) by e-maill. We will contact you to confirm receipt of your request.

Request for Service form

Data sheets


Overview of rates

  • FVB/NJ Strain Pronuclear or Cytoplasm Injection: $4545 per construct + per diem
  • C57BL/6J Strain Pronuclear or Cytoplasm Injection: $5050 per construct + per diem
  • ES Cell Blastocyst Injection: $2360 + per diem
  • Sperm Cryopreservation: $505/up to 3 lines
  • In Vitro Fertilization (IVF): $1010 + mouse cost (for C57BL/6J, the cost is $645) + per diem
  • Specialized Services $505/day + mouse purchase + per diem

Overview video

Mouse Genetics - Masonic Cancer Center

Chimera mouse with an albino mouse

New service

MGL is working with Genome Editing Shared Resources (GESR) to provide a full service for your CRISPR/Cas9 project (to produce mutant mice).  

Please contact either MGL coordinator Yun You (yyou@umn.edu or 612-626-5825) or GESR coordinator Brian Ruis (ruisx001@umn.edu or 612-625-1484) to start.

Note: The CRISPR/Cas9 has revolutionized genome editing, however, there are difference between genome editing in cell lines versus genome editing to make mutant mice. CRISPR/Cas9 is the best to making simple gene knock-out.  Injection of CRISPR/Cas9 reagents directly to mouse zygotes (fertilized eggs) can produce indels in the resulting pups/mice with high efficiency, even bi-allelic mutations.  Using CRISPR/Cas9 to insert LoxP elements to flank a genomic region is not recommended as of now.  MGL recommends to consult experts in the field before making any decision regarding which route to take to make your mutant mice.

View rates and request our services

Contact us

Anindya Bagchi, Ph.D., Director
612-625-2132
bagch005@umn.edu

Yun You, Ph.D., Coordinator
612-626-5825
yyou@umn.edu

Mail us:

Mouse Genetics Laboratory
University of Minnesota
2231 6th St. SE
Minneapolis, MN, 55455

Visit us:

Mouse Genetics Laboratory
Cancer and Cardiovascular Research Building
2231 6th St. SE
Minneapolis, MN, 55455