Mouse Genetics Laboratory

Chimera mouse with 2 different eye color

Mouse Genetics Laboratory (MGL), acting as a Research Resource for the Office of Academic Clinical Affairs, provides a comprehensive range of mouse embryo manipulation services.

Housed in the Cancer Cardiovascular Research Building (CCRB), MGL offers microinjection, mouse embryo manipulation, tissue culture, and administration capabilities in conjunction with dedicated animal housing space. 

Download the Request for Services form

Download the Request for Service form

Service & Cost Overview

ServiceDescriptionNon-Cancer Center Member Cost EstimateCancer Center Member Cost Estimate
CRISPR or DNA transgenicsDirect zygote injection or electroporation$4545 (FVB) or $5050 (non-FVB) + per diem$2272.50 (FVB) or $2525 (non-FVB) + per diem
ES cell injection to make chimera miceESC or iPSC injection into blastocyst $2360/mutation + per diem$1180/mutation + per diem
Sperm cryoCryopreservation of sperm$505/1-3 lines/PI (Lab)$252.50/1-3 lines/PI (Lab)
In Vitro Fertilization (IVF)Fertilization of eggs and embryo transfer$1655/line + per diem$827.50/line + per diem
Recover mice from frozen embryoEmbryo transfer$505 + mouse cost + per diem$252.50 + mouse cost + per diem
Special serviceProject specific, procedure varies.$505/day + mouse cost + per diem$252.50/day + mouse cost + per diem

Overview video

Mouse Genetics - Masonic Cancer Center

Creating mutant mice by CRISPR/Cas9 methods

Working with Genome Engineering Shared Resource (GESR) MGL can provide full service for your CRISPR/Cas9 project to produce mutant mice. We have successfully created mutant mice with simple gene KO (indels), large deletions (more than 1 Kb), point mutations, conditional KO alleles (inserting 2 LoxP elements), and knock-in DNA fragments (> 4 Kb).

Work flow and cost

To start, please contact either MGL coordinator Yun You ( or GESR coordinator Brian Ruis (


Note: CRISPR/Cas9 approach is the method of choice to make simple gene knock-out and precision point mutations. More recently, substancial strides were made to enhance the rate of success in making conditional alleles (inserting LoxP, Frt, etc). Please contact MGL to discuss your project.

Contact us

Timothy Hallstrom, Ph.D., Director

Yun You, Ph.D., Coordinator


Mouse Genetics Laboratory (MGL)
Cancer and Cardiovascular Research Building
2231 6th St. SE
Minneapolis, MN, 55455