Mouse Genetics Laboratory
Mouse Genetics Laboratory (MGL), acting as a Research Resource for the Academic Health Center, provides a comprehensive range of mouse embryo manipulation services.
Housed in the Cancer Cardiovascular Research Building (CCRB), MGL offers microinjection, mouse embryo manipulation, tissue culture, and administration capabilities in conjunction with dedicated animal housing space.
Service Overview
| Service | Description | Cost Estimate |
|---|---|---|
| CRISPR or DNA transgenics | Direct zygote microinjection | $4545 (FVB) or $5050 (non-FVB) + per diem |
| ES cell injection to make chimera mice | ES cell injection into blastocyst | $2360/mutation + per diem |
| Sperm cryo | Cryopreservation of sperm | $505/1-3 lines |
| In Vitro Fertilization (IVF) | Fertilization of eggs and embryo transfer | $1655/line + per diem |
| Recover mice from frozen embryo | Embryo transfer | $505 + mouse cost + per diem |
| Special service | Project specific, procedure varies. | $505/day + mouse cost + per diem |
Down load the Request for Services form
Overview video
Creating mutant mice by CRISPR/Cas9 injection
Working with Genome Engineering Shared Resource (GESR) MGL can provide a full service for your CRISPR/Cas9 project to produce mutant mice.
To start, please contact either MGL coordinator Yun You (yyou@umn.edu or GESR coordinator Brian Ruis (ruisx001@umn.edu.
Note: CRISPR/Cas9 approach is the method of choice to make simple gene knock-out. It is also very useful to generate point mutations. However, it is still a work in progress to efficiently insert two LoxP elements to flank a genomic region (conditional allele) or insert large DNA fragment (GFP, etc.). Please contact MGL to discuss your project.
Contact us
Timothy Hallstrom, Ph.D., Director
612-626-2905
halls026@umn.edu
Yun You, Ph.D., Coordinator
612-626-5825
yyou@umn.edu
Location:
Mouse Genetics Laboratory (MGL)
Cancer and Cardiovascular Research Building
2231 6th St. SE
Minneapolis, MN, 55455