Mouse Genetics Laboratory
Mouse Genetics Laboratory (MGL), acting as a Research Resource for the Academic Health Center, provides a comprehensive range of mouse embryo manipulation services.
Housed in the Cancer Cardiovascular Research Building (CCRB), MGL offers microinjection, mouse embryo manipulation, tissue culture, and administration capabilities in conjunction with dedicated animal housing space.
|CRISPR or DNA transgenics||Direct zygote microinjection||$4545 (FVB) or $5050 (non-FVB) + per diem|
|ES cell injection to make chimera mice||ES cell injection into blastocyst||$2360/mutation + per diem|
|Sperm cryo||Cryopreservation of sperm||$505/1-3 lines|
|In Vitro Fertilization (IVF)||Fertilization of eggs and embryo transfer||$1655/line + per diem|
|Recover mice from frozen embryo||Embryo transfer||$505 + mouse cost + per diem|
|Special service||Project specific, procedure varies.||$505/day + mouse cost + per diem|
Creating mutant mice by CRISPR/Cas9 injection
Note: CRISPR/Cas9 approach is the method of choice to make simple gene knock-out. It is also very useful to generate point mutations. However, it is still a work in progress to efficiently insert two LoxP elements to flank a genomic region (conditional allele) or insert large DNA fragment (GFP, etc.). Please contact MGL to discuss your project.