Instrumentation & Locations

Instrumentation and Locations

Self-Serve Analyzers

Self-Serve Spectral Analyzers

Cytek Aurora | Configuration | Fluorescent Guide (Cytek)

  • 3 lasers: 100mW Violet (405nm), 50mW Blue (488nm) and 80mW Red (640nm) spatially-separated laser sources
  • 38 fluorescent parameters (16V-14B-8R)
  • Semiconductor array for each laser enabling more efficient spectrum capture in the 420nm-830nm range. No filter changes required for any fluorochromes excited by the 405nm, 488nm and 640nm lasers.
  • Pulse Area and Height of all parameters; Pulse Width of scatter parameters and one fluorescent parameter per laser

Location: 1-209 Cancer and Cardiovascular Research Building (CCRB)

NOTE: This instrument requires additional training to use. For training, please submit the UFCR Aurora Training Request form. Completion of UFCR Digital Lecture and Lab Training and Post-Training Validation are prerequisites for Cytek Aurora training.

Supporting Documentation:

Self-Serve Conventional Analyzers

BD LSR II/Fortessa/Canto

BD LSR II H1160 | Configuration | Fluorescent Guide

  • 20mW Blue (488nm), 17mW Red (633nm), 25mW Violet (405nm) spatially-separated laser sources
  • 14 fluorescent parameters (5/3/6)

Location: 621 Masonic Cancer Research Building (MCRB)

BD LSR II H1010 | Configuration | Fluorescent Guide

  • 20mW Blue (488nm), 40mW Red (640nm), 50mW Violet (405nm) and 40mW Yellow-Green (561nm) spatially-separated laser sources
  • 14 fluorescent parameters (2/3/6/3)

Location: 2-289 Wallin Medical Biosciences Building (WMBB)

BD LSR II H4710 | Configuration | Fluorescent Guide

  • 20mW Blue (488nm), 40mW Red (640nm), 25mW Violet (405nm) spatially-separated laser sources
  • 14 fluorescent parameters (5/3/6)

Location: 621 Masonic Cancer Research Building (MCRB)

BD LSR II H4760 | Configuration | Fluorescent Guide

  • 20mW Blue (488nm), 17mW Red (633nm) and 25mW Violet (405nm) spatially-separated laser sources
  • 14 fluorescent parameters (5/3/6)

Location: 1-209 Cancer and Cardiovascular Research Building (CCRB)

BD Fortessa H1770 | Configuration | Fluorescent Guide

  • 50mW Blue (488nm), 40mW Red (640nm), 100mW Violet (405nm), 40mW Yellow-Green (561nm) and 20mW UV (355nm) spatially-separated laser sources
  • 18 fluorescent parameters (2/3/6/3/4)

Location: 2-289 Wallin Medical Biosciences Building (WMBB)

BD Fortessa X-30 H0081 | Configuration | Fluorescent Guide

  • 50mW Blue (488nm), 100mW Red (640nm), 100mW Violet (405nm), 100mW Yellow-Green (561nm) and 20mW UV (355nm) spatially-separated laser sources
  • 28 fluorescent parameters (5/3/8/5/7)
  • Important Fluidic System Changes. Read more about the BD LSRFortessa H0081.

Location: 1-209 Cancer and Cardiovascular Research Building (CCRB)

BD Fortessa X-20 | Configuration | Fluorescent Guide

  • 50mW Blue (488nm), 100mW Red (640nm), 50mW Violet (405nm), 50mW Yellow-Green (561nm) and 15mW UV (355nm) spatially-separated laser sources
  • 18 fluorescent parameters (2/3/6/3/4)

Location: 1-209 Cancer and Cardiovascular Research Building (CCRB)

BD FACSCanto A V07300428 | Configuration | Fluorescent Guide

  • 20mW Blue (488nm), 50mW Violet (405nm) and 17mW Red (633nm) spatially-separated laser sources
  • 10 fluorescent parameter instrument (4/3/3)

Location: 621 Masonic Cancer Research Building (MCRB)

Cell Sorters

BD FACSAria II P07800142 (BSL2) | Configuration

  • High or low speed sorter
  • 50mw Blue (488nm), 100mW Red (640nm), 100 mW Violet (405nm) and 100mW Yellow-Green (561nm), spatially-separated laser sources
  • 14 fluorescent parameters (2/3/6/3)

Features:

  • Nozzle Sizes: 70um, 85um, 100um and 130um
  • 4-way sorting w/ sample cooling
  • Single cell plate sorting
  • Flexible sample tube size and cooling
  • Adjustable optical emission elements   

This custom instrument is installed inside a Baker Bio-Protect biohazard containment cabinet with an internal Aerosol Management System.

Location: 1-209A Cancer and Cardiovascular Research Building (CCRB)

BD FACSAria II P0287 (BSL2)Configuration

  • High or low speed sorter
  • 50mw Blue (488nm), 40mW Red (640nm), 100mW Violet (405nm) and 100mW Yellow Green (561nm), spatially-separated laser sources
  • 14 fluorescent parameters (2/3/6/3)

Features:

  • Nozzle Sizes: 70um, 85um, 100um and 130um
  • 4-way sorting w/ sample cooling
  • Single cell plate sorting
  • Flexible sample tube size and cooling
  • Adjustable optical emission elements

Location: 695 Masonic Cancer Research Building (MCRB)

BD FACSAria II P69500132 (BSL2E) | Configuration

  • High or low speed sorter
  • 50mw Blue (488nm), 40mW Red (640nm) and 100mW Violet (405nm), 50mW Yellow-Green (561nm) spatially-separated laser sources
  • 14 fluorescent parameters (2/3/6/3)

Features:

  • Nozzle Sizes: 70um, 85um, 100um and 130um
  • 4-way sorting w/ sample cooling
  • Single cell plate sorting
  • Flexible sample tube size and cooling
  • Adjustable optical emission elements
  • FSC PMT module for small particle detection

This custom instrument is installed inside a Baker Bio-Protect biohazard containment cabinet with an internal Aerosol Management System. A separate remote computer terminal located outside of the BL2 + Lab provides ability for the customer to safely interact with the sort operator for proper setup and monitoring of the procedure.

Location: 2-­128A Microbiology Research Facility (MRF)

Data Analysis Resources

NOTE: Due to low usage and the adoption of the FlowJo Portal system, data analysis workstations have been removed from service. Alternative options offered by the UFCR are listed below.

FlowJo Portal License System

To increase accessibility and minimize the cost for FlowJo software analysis, the UFCR has created a FlowJo Portal license group. For approximately $20/month per license, FlowJo Portal users will be able to access the most up-to-date version of FlowJo on any internet-connected computer. Those interested in joining the Portal group should create a FlowJo Portal account and then submit a FlowJo Portal Purchase or Change Request form. FlowJo Portal accounts for this group must use a valid email account with @umn.edu domain. All new requests will be processed monthly on the first business day of the month. The cost will be prorated based on the remaining term of the annual license account, which renews in October. Additional information and instructions on creating a FlowJo Portal account and accessing the Portal can be found in the documents below or at the FlowJo Portal or FlowJo Portal Documentation websites.

FlowJo Hardware Dongle Lending

2 UFCR-owned FlowJo dongles, 1 each at MCRB and CCRB, are available for active UFCR customers to borrow at no charge for up to 5 business days. If you are interested in borrowing a dongle, please submit this form and the UFCR will contact you to arrange pick-up of the requested dongle. The borrower is responsible for returning the dongle when it is due during regular UFCR business hours (9AM-5PM). Damaged or lost dongles will incur a full replacement fee at the current academic purchase price.