Instrumentation & Locations

Instrumentation and Locations

Self-Serve Analyzers

Self-Serve Spectral Analyzers

Cytek Aurora | Configuration | Fluorescent Guide (Cytek)

  • 3 lasers: 100mW Violet (405nm), 50mW Blue (488nm) and 80mW Red (640nm) spatially-separated laser sources
  • 38 fluorescent parameters (16V-14B-8R)
  • Semiconductor array for each laser enabling more efficient spectrum capture in the 420nm-830nm range. No filter changes required for any fluorochromes excited by the 405nm, 488nm and 640nm lasers.
  • Pulse Area and Height of all parameters; Pulse Width of scatter parameters and one fluorescent parameter per laser

Location: 1-209 Cancer and Cardiovascular Research Building (CCRB)

Supporting Documentation:

Self-Serve Conventional Analyzers

BD LSR II/Fortessa/Canto

BD LSR II H1160 | Configuration | Fluorescent Guide

  • 20mW Blue (488nm), 17mW Red (633nm), 25mW Violet (405nm) spatially-separated laser sources
  • 14 fluorescent parameters (5/3/6)

Location: 621 Masonic Cancer Research Building (MCRB)

BD LSR II H1010 | Configuration | Fluorescent Guide

  • 20mW Blue (488nm), 40mW Red (640nm), 50mW Violet (405nm) and 40mW Yellow-Green (561nm) spatially-separated laser sources
  • 14 fluorescent parameters (2/3/6/3)

Location: 2-289 Wallin Medical Biosciences Building (WMBB)

BD LSR II H4710 | Configuration | Fluorescent Guide

  • 20mW Blue (488nm), 40mW Red (640nm), 25mW Violet (405nm) spatially-separated laser sources
  • 14 fluorescent parameters (5/3/6)

Location: 621 Masonic Cancer Research Building (MCRB)

BD LSR II H4760 | Configuration | Fluorescent Guide

  • 20mW Blue (488nm), 17mW Red (633nm) and 25mW Violet (405nm) spatially-separated laser sources
  • 14 fluorescent parameters (5/3/6)

Location: 1-209 Cancer and Cardiovascular Research Building (CCRB)

BD Fortessa H1770 | Configuration | Fluorescent Guide

  • 50mW Blue (488nm), 40mW Red (640nm), 100mW Violet (405nm), 40mW Yellow-Green (561nm) and 20mW UV (355nm) spatially-separated laser sources
  • 18 fluorescent parameters (2/3/6/3/4)

Location: 2-289 Wallin Medical Biosciences Building (WMBB)

BD Fortessa H0081 | Configuration | Fluorescent Guide

  • 50mW Blue (488nm), 100mW Red (640nm), 100mW Violet (405nm), 100mW Yellow-Green (561nm) and 20mW UV (355nm) spatially-separated laser sources
  • 18 fluorescent parameters (2/3/6/3/4)
  • Important Fluidic System Changes. Read more about the BD LSRFortessa H0081.

Location: 1-209 Cancer and Cardiovascular Research Building (CCRB)

BD Fortessa X-20 | Configuration | Fluorescent Guide

  • 50mW Blue (488nm), 100mW Red (640nm), 50mW Violet (405nm), 50mW Yellow-Green (561nm) and 15mW UV (355nm) spatially-separated laser sources
  • 18 fluorescent parameters (2/3/6/3/4)

Location: 1-209 Cancer and Cardiovascular Research Building (CCRB)

BD FACSCanto A V07300428 | Configuration | Fluorescent Guide

  • 20mW Blue (488nm), 50mW Violet (405nm) and 17mW Red (633nm) spatially-separated laser sources
  • 10 fluorescent parameter instrument (4/3/3)

Location: 621 Masonic Cancer Research Building (MCRB)

Cell Sorters

BD FACSAria II P07800142 (BSL2) | Configuration

  • High or low speed sorter
  • 50mw Blue (488nm), 100mW Red (640nm), 100 mW Violet (405nm) and 100mW Yellow-Green (561nm), spatially-separated laser sources
  • 14 fluorescent parameters (2/3/6/3)

Features:

  • Nozzle Sizes: 70um, 85um, 100um and 130um
  • 4-way sorting w/ sample cooling
  • Single cell plate sorting
  • Flexible sample tube size and cooling
  • Adjustable optical emission elements   

This custom instrument is installed inside a Baker Bio-Protect biohazard containment cabinet with an internal Aerosol Management System.

Location: 1-209A Cancer and Cardiovascular Research Building (CCRB)

BD FACSAria II P0287 (BSL2)Configuration

  • High or low speed sorter
  • 50mw Blue (488nm), 40mW Red (640nm), 100mW Violet (405nm) and 100mW Yellow Green (561nm), spatially-separated laser sources
  • 14 fluorescent parameters (2/3/6/3)

Features:

  • Nozzle Sizes: 70um, 85um, 100um and 130um
  • 4-way sorting w/ sample cooling
  • Single cell plate sorting
  • Flexible sample tube size and cooling
  • Adjustable optical emission elements

Location: 695 Masonic Cancer Research Building (MCRB)

BD FACSAria II P69500132 (BSL2E) | Configuration

  • High or low speed sorter
  • 50mw Blue (488nm), 40mW Red (640nm) and 100mW Violet (405nm), 50mW Yellow-Green (561nm) spatially-separated laser sources
  • 14 fluorescent parameters (2/3/6/3)

Features:

  • Nozzle Sizes: 70um, 85um, 100um and 130um
  • 4-way sorting w/ sample cooling
  • Single cell plate sorting
  • Flexible sample tube size and cooling
  • Adjustable optical emission elements
  • FSC PMT module for small particle detection

This custom instrument is installed inside a Baker Bio-Protect biohazard containment cabinet with an internal Aerosol Management System. A separate remote computer terminal located outside of the BL2 + Lab provides ability for the customer to safely interact with the sort operator for proper setup and monitoring of the procedure.

Location: 2-­128A Microbiology Research Facility (MRF)

Analysis Workstations Locations

621 Masonic Cancer Research Building (MCRB)

  • BD FACSDiva v8.0.1
  • Flowjo v7.6.5
  • FlowjoX v10.4.2
  • BD Accuri C6

1-209 Cancer and Cardiovascular Research Building (CCRB)

  • BD FACSDiva v8.0.1
  • Flowjo v7.6.5
  • FlowjoX v10.4.2
  • BD Accuri C6